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blood_samples [2023/02/14 09:35] – rewritten for 3OR, Quality Control split to its own dedicated page kevinblood_samples [2026/09/11 07:41] (current) – [Centrifuging] rick
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 ====== Blood samples ====== ====== Blood samples ======
  
-Blood samples are collected in a variety of different blood tubes such as: heparin, EDTA, sodiumfluoride, and gel separation tubes. Blood collected in heparin- and sodiumfluoride tubes are used to perform [[blood_analyses|analyses]] of clinical and haematological parameters (including LDL/HDL cholesterol, HbA1c, and triglycerides) and are therefore no longer available for release. The results of these [[blood_analyses|analyses]] can be obtained upon request.\\ +Blood samples are collected in a variety of different blood tubes. A subset of tubes is used for [[blood_analyses|analyzing]] of different parameters including: DL/HDL cholesterol, HbA1c and triglycerides, and are thus no longer available for release. The results of these [[blood_analyses|analyses]] can be obtained upon request. Plasma and serum are stored in the Lifelines Biobank and can be requested for research purposes. DNA samples are [[dna_samples|isolated]] from either wholeblood or buffycoat based on the assessment round, and can likewise be requested for research purposes. 
-Plasma/buffycoat samples derived from EDTA collection tubes and serum samples derived from gel separated tubes are stored in the Lifelines Biobank and can be requested for research purposes.\\ +Furthermore [[cryopreserved_whole_blood|wholeblood]] samples preserved in liquid nitrogen can also be requested for researchers.
-DNA samples are extracted from the buffycoat fractions and can likewise be requested for research purposes.\\+
 \\ \\
 From collection to storage, Lifelines adheres to [[sample quality|stringent quality control]] protocols to ensure that the samples are fit for use in all standard blood analysis protocols. Standard operating procedures for all the steps described below can be provided by Lifelines on request.\\ From collection to storage, Lifelines adheres to [[sample quality|stringent quality control]] protocols to ensure that the samples are fit for use in all standard blood analysis protocols. Standard operating procedures for all the steps described below can be provided by Lifelines on request.\\
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 ===== Current Study Protocol ===== ===== Current Study Protocol =====
  
-Please note that the text below describes the procedures as they are employed during the ongoing [[3A|third]] assessment. The [[1A|baseline]]- and [[2A|second]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.+Please note that the text below describes the procedures as they are employed during the ongoing [[4A|fourth]] assessment. The [[1A|baseline]]-, [[2A|second]]- and [[3A|third]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.
  
 ===== Sample Collection ===== ===== Sample Collection =====
  
 Blood was typically collected between the hours of 07:00 and 10:00, and participants were asked to refrain from consuming any food or liquids (with the exception of water) after 22:00 on the evening preceding visit 2.\\ Blood was typically collected between the hours of 07:00 and 10:00, and participants were asked to refrain from consuming any food or liquids (with the exception of water) after 22:00 on the evening preceding visit 2.\\
-Blood was primarily collected through venopuncture of the median cubical vein of the right arm using Venoject® needles in combination with [[https://www.bd.com/en-us/products-and-solutions/products/product-families/bd-vacutainer-blood-collection-tubes#productsaccessories|Vacutainer®]] blood collection tubes, although butterfly needles and venopuncture of other locations (such as the left arm or the wrist) have also been employed if the previous was not an option.+Blood was primarily collected through venopuncture of the median cubical vein using Venoject® needles in combination with [[https://www.bd.com/en-us/products-and-solutions/products/product-families/bd-vacutainer-blood-collection-tubes#productsaccessories|Vacutainer®]] blood collection tubes, although butterfly needles and venopuncture of other locations such as the wrist have also been employed if the previous was not an option.
  
 ==== Types of Blood Collection Tubes ==== ==== Types of Blood Collection Tubes ====
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 ===== Sample Transport ===== ===== Sample Transport =====
  
-After collection the blood tubes are placed in a refrigerated (using ice packs), insulated transport box. The temperatures in these boxes are continuously monitored ([[logtag|LogTag TRIX-8]]) as part of our [[sample_quality|quality control]] procedures. These transport boxes are then shipped to the Lifelines laboratory, typically arriving between the hours of 10:00–12:00.+After collection the blood tubes are placed in a refrigerated and insulated transport box. The temperatures in these boxes are continuously monitored ([[logtag|LogTag TRIX-8]]) as part of our [[sample_quality|quality control]] procedures. These transport boxes are then shipped to the Lifelines laboratory.
  
 ===== Sample Reception ===== ===== Sample Reception =====
  
 Upon arrival at the Lifelines laboratory the blood collection tubes are divided into two distinct categories: Upon arrival at the Lifelines laboratory the blood collection tubes are divided into two distinct categories:
-  * Blood collection tubes meant for diagnostic testing are refrigerated (at 4 degrees celsius) and sent onwards to the UMCG in a refrigerated transport box. +  * Blood collection tubes meant for diagnostic testing are refrigerated (at 4 degrees Celsius) and sent onwards to the UMCG in a refrigerated transport box. 
-  *  Blood samples to be stored by Lifelines are either immediately centrifuged (according to the protocols detailed below) or refrigerated (at 4 degrees celsius) until this is possible.+  *  Blood samples to be stored by Lifelines are either immediately centrifuged (according to the protocols detailed below) or refrigerated (at 4 degrees Celsius) until this is possible.
 The temperature measurements taken during transport are reviewed and saved to our labotatory information management system (LIMS). The temperature measurements taken during transport are reviewed and saved to our labotatory information management system (LIMS).
  
 ==== Centrifuging ==== ==== Centrifuging ====
  
-  * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees celsius, no brake +  * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees Celsius, full brake 
-  * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees celsius, no brake+  * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees Celsius, full brake
  
 ===== Sample Processing ===== ===== Sample Processing =====
  
-Blood (plasma) and blood (serum) samples are divided into 900 μl fractions by the [[https://lifesciences.tecan.com/freedom-evo-packages|Tecan Freedom EVOware® robot]], a semi-automated system that combines pipetting, robotics, and scheduling of multiple devices.\\+Blood (plasma) and blood (serum) samples are divided into 900 μl fractions by a Hamilton robotics liquid handler, a semi-automated system that combines pipetting, robotics, and scheduling of multiple devices.\\
 These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\ These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\
 During sample processing special care is given to note deviations, such as hemolysis and lipemia, and to register these in our LIMS.\\ During sample processing special care is given to note deviations, such as hemolysis and lipemia, and to register these in our LIMS.\\
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 ===== Sample Storage ===== ===== Sample Storage =====
  
-Aliquots are kept refrigerated (at 4 degrees celsius) until they can be moved into a [[sample_storage|cryofreezer]] (-80 degrees Celsius), which is typically within 10 hours after sample collection.\\+Aliquots are kept refrigerated (at 4 degrees Celsius) until they can be moved into a [[sample_storage|cryofreezer]] (-80 degrees Celsius), which is typically within 10 hours after sample collection.\\
  
blood_samples.1676367359.txt.gz · Last modified: (external edit)