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blood_samples [2023/03/23 14:19] kevin [Sample Reception] |
blood_samples [2025/02/05 14:49] (current) |
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Upon arrival at the Lifelines laboratory the blood collection tubes are divided into two distinct categories: | Upon arrival at the Lifelines laboratory the blood collection tubes are divided into two distinct categories: | ||
* Blood collection tubes meant for diagnostic testing are refrigerated (at 4 degrees Celsius) and sent onwards to the UMCG in a refrigerated transport box. | * Blood collection tubes meant for diagnostic testing are refrigerated (at 4 degrees Celsius) and sent onwards to the UMCG in a refrigerated transport box. | ||
- | * Blood samples to be stored by Lifelines are either immediately centrifuged (according to the protocols detailed below) or refrigerated (at 4 degrees celsius) until this is possible. | + | * Blood samples to be stored by Lifelines are either immediately centrifuged (according to the protocols detailed below) or refrigerated (at 4 degrees Celsius) until this is possible. |
The temperature measurements taken during transport are reviewed and saved to our labotatory information management system (LIMS). | The temperature measurements taken during transport are reviewed and saved to our labotatory information management system (LIMS). | ||
==== Centrifuging ==== | ==== Centrifuging ==== | ||
- | * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees celsius, no brake | + | * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees Celsius, no brake |
- | * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees celsius, no brake | + | * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees Celsius, no brake |
===== Sample Processing ===== | ===== Sample Processing ===== | ||
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===== Sample Storage ===== | ===== Sample Storage ===== | ||
- | Aliquots are kept refrigerated (at 4 degrees celsius) until they can be moved into a [[sample_storage|cryofreezer]] (-80 degrees Celsius), which is typically within 10 hours after sample collection.\\ | + | Aliquots are kept refrigerated (at 4 degrees Celsius) until they can be moved into a [[sample_storage|cryofreezer]] (-80 degrees Celsius), which is typically within 10 hours after sample collection.\\ |