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blood_samples [2026/09/04 13:01] – rickblood_samples [2026/09/11 07:41] (current) – [Centrifuging] rick
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 ===== Current Study Protocol ===== ===== Current Study Protocol =====
  
-Please note that the text below describes the procedures as they are employed during the ongoing [[3A|third]] assessment. The [[1A|baseline]]- and [[2A|second]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.+Please note that the text below describes the procedures as they are employed during the ongoing [[4A|fourth]] assessment. The [[1A|baseline]]-, [[2A|second]]- and [[3A|third]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.
  
 ===== Sample Collection ===== ===== Sample Collection =====
  
 Blood was typically collected between the hours of 07:00 and 10:00, and participants were asked to refrain from consuming any food or liquids (with the exception of water) after 22:00 on the evening preceding visit 2.\\ Blood was typically collected between the hours of 07:00 and 10:00, and participants were asked to refrain from consuming any food or liquids (with the exception of water) after 22:00 on the evening preceding visit 2.\\
-Blood was primarily collected through venopuncture of the median cubical vein of the right arm using Venoject® needles in combination with [[https://www.bd.com/en-us/products-and-solutions/products/product-families/bd-vacutainer-blood-collection-tubes#productsaccessories|Vacutainer®]] blood collection tubes, although butterfly needles and venopuncture of other locations (such as the left arm or the wrist) have also been employed if the previous was not an option.+Blood was primarily collected through venopuncture of the median cubical vein using Venoject® needles in combination with [[https://www.bd.com/en-us/products-and-solutions/products/product-families/bd-vacutainer-blood-collection-tubes#productsaccessories|Vacutainer®]] blood collection tubes, although butterfly needles and venopuncture of other locations such as the wrist have also been employed if the previous was not an option.
  
 ==== Types of Blood Collection Tubes ==== ==== Types of Blood Collection Tubes ====
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 ===== Sample Transport ===== ===== Sample Transport =====
  
-After collection the blood tubes are placed in a refrigerated (using ice packs), insulated transport box. The temperatures in these boxes are continuously monitored ([[logtag|LogTag TRIX-8]]) as part of our [[sample_quality|quality control]] procedures. These transport boxes are then shipped to the Lifelines laboratory, typically arriving between the hours of 10:00–12:00.+After collection the blood tubes are placed in a refrigerated and insulated transport box. The temperatures in these boxes are continuously monitored ([[logtag|LogTag TRIX-8]]) as part of our [[sample_quality|quality control]] procedures. These transport boxes are then shipped to the Lifelines laboratory.
  
 ===== Sample Reception ===== ===== Sample Reception =====
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 ==== Centrifuging ==== ==== Centrifuging ====
  
-  * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees Celsius, no brake +  * 10.0 mL K2E (EDTA) 18.0mg Plus blood collection samples | 15 minutes @ 2500RCF and 4 degrees Celsius, full brake 
-  * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees Celsius, no brake+  * 10.0 mL SST II Advance blood collection samples | 10 minutes @ 2500RCF and 18 degrees Celsius, full brake
  
 ===== Sample Processing ===== ===== Sample Processing =====
  
-Blood (plasma) and blood (serum) samples are divided into 900 μl fractions by the [[https://lifesciences.tecan.com/freedom-evo-packages|Tecan Freedom EVOware® robot]], a semi-automated system that combines pipetting, robotics, and scheduling of multiple devices.\\+Blood (plasma) and blood (serum) samples are divided into 900 μl fractions by a Hamilton robotics liquid handler, a semi-automated system that combines pipetting, robotics, and scheduling of multiple devices.\\
 These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\ These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\
 During sample processing special care is given to note deviations, such as hemolysis and lipemia, and to register these in our LIMS.\\ During sample processing special care is given to note deviations, such as hemolysis and lipemia, and to register these in our LIMS.\\
blood_samples.1788526881.txt.gz · Last modified: by rick