cryopreserved_white_blood_cells
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cryopreserved_white_blood_cells [2019/08/28 15:10] hilde |
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| - | {{:136091-004-c22a66a2.jpg?200|}}{{:cryotube.png?200|}} | ||
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| - | 1,5 ml prepared blood cells( Fetal Calf serum,DMSO, Heparin Blood ) in cryotube. | ||
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| - | To preserve PBMCs at ultra-low temperatures in liquid nitrogen, dimethyl sulfoxide (DMSO) is used as a cryoprotectant to reduce the formation of ice crystals and prevent cell damage. However, DMSO is toxic to cells at higher temperature and must be washed off immediately once cells are thawed. To freeze, freshly isolated PBMCs are resuspended to 5-10 x 106 cells/mL in freezing medium containing 10-20% DMSO and 40% fetal bovine serum | ||
cryopreserved_white_blood_cells.1566997811.txt.gz ยท Last modified: 2025/02/05 14:49 (external edit)
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