These peripheral blood mononuclear cells (PMBC's) are leukocytes isolated from whole blood and comprise of monocytes (macrophage precursors), lymphocytes (both B and T cells), and a small percentage of other immune cells, such as dendritic cells.

Lifelines isolated PMBC's from freshly drawn blood from xxx participants during 1A Visit 2.
Freshly isolated PBMCs were resuspended to 5-10 x 10^6 cells/mL in freezing medium containing 10-20% dimethyl sulfoxide (DMSO) and 40% fetal bovine serum, and were cryopreserved at ultra-low temperatures in liquid nitrogen to remain viable for decades with no significant changes to viability or functionality.
Note that DMSO, used as a cryoprotectant to reduce the formation of ice crystals and prevent cell damage, is toxic to cells at higher temperature and must be washed off immediately once cells are thawed.

After a careful thawing procedure, cryopreserved (c-) PBMCs can be stimulated or treated with various immunomodulatory agents or drugs in vitro.