On top of the general assessments, additional assessments may be performed in selected groups of Lifelines participants (e.g. “subcohorts”) in order to collect additional questionnaire data, measurements and/or biomaterials.
Additional assessments are performed at the initiative of (and in collaboration with) external research groups, and an embargo period for use of additional data by third parties may apply.

The following additional measurements/sample collections have been performed:

• DAG1, a.k.a. DEEP, analysis of microbiome and methylome in samples of ca. ~1.500 participants
• DAG2, the assessment of whole genome + fecal microbiome in samples of ca. 165 participants
• DAG3, the assessment of the microbiome in samples of ca. ~9.000 participants
• DAG4, the follow-up to DAG3
• DAG5, the follow-up to DAG4
• EYEL, the assessment of glaucoma in elderly participants (EyeLife)
• GWAS, the genotyping of ~15.000 participants
• UGLI, the genotyping of ~38.500 (UGLI-1) plus ~28,000 (UGLI-2) participants
• HAIR, the collection & analysis of scalp hair samples
• IMAL, the assessment of CT scans of the lungs & hearts of ~12.000 participants (ImaLife)
• MYST, the use of quantified-self devices in ~100 participants
• PGPX, the use of a pharmacogenetic passport in an app by ~1000 participants
• NEXT, the assessment of ~1500 pregnant Lifelines participants (currently an independent project)
• SALT, additional urine analyses and questionnaire on salt usage
• VESP, the assessment of fertility, sperm quality and plasma hormones in ~200 participants (Vesper)

The following additional questionnaires have been performed:

• ACNQ, a questionnaire on acne
• ACTQ, a questionnaire on daily activities and leisure activities (DALAS)
• ADHQ, a questionnaire on ADHD and related behavioral problems
• AUTQ, a questionnaire on autistic traits
• BIGQ, a questionnaire on values and beliefs around big data use
• BLUQ, a questionnaire on children's exposure to blue light
• COVQ, a series of questionnaires during the covid-19 pandemic
• DCDQ, a questionnaire on motoric development
• DEPQ, a questionnaire on lifetime depression (BIONIC-I)
• DEAQ, a questionnaire on lifetime depression and anxiety (BIONIC-II)
• DIVQ, a questionnaire on well-being and the kinship network after parental divorce
• EARQ, a questionnaire on experiences with earthquakes
• INFQ, a questionnaire on informal care (ICAS)
• MAWQ, a questionnaire on menopause and work
• MCFQ, a questionnaire on myalgic encephalomyelitis/chronic fatigue syndrome
• MHDQ, a questionnaire on the links between (familial) mental health and diabetes
• MUSQ, a questionnaire on language use and musical training
• OPBQ, a questionnaire on optimism, parental bonding and emotion regulation
• RELQ, a questionnaire on the cognitive benefits of regional language use
• ROAQ, a questionnaire on female reproductive health (ROAHD)
• SHIQ, a questionnaire on shiftwork and irregular working hours
• SKIQ, a questionnaire on the skin conditions hidradentitis supperative and eczema
• SPEQ, a questionnaire on speech, language, and music